outer mitochondrial membrane 20  (Proteintech)

Journal: Journal of Pharmaceutical Analysis

Article Title: Exploring the impact of the liver-intestine-brain axis on brain function in non-alcoholic fatty liver disease

doi: 10.1016/j.jpha.2024.101077

Figure Lengend Snippet: Investigation of mitochondrial dysfunction and microglial activation mechanisms. (A, B) Measurement of reactive oxygen species (ROS) accumulation in cells by dihydroethidium (DHE) assay and monitoring by fluorescence microscopy (A) and statistical chart (B). (C, D) Detection of mitochondrial membrane potential (MMP) in BV2 cells using 5,5',6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimidazolylcarbocyanine iodide (JC-1) staining (C) and statistical chart (D). (E, F) Performance of Western blot to measure the expression levels of autophagy markers in BV2 cells (E) and statistical chart (F). (G, H) Utilization of immunofluorescence staining to determine the expression level of microtubule-associated protein 1a/1b-light chain 3 (LC3) in BV2 cells (G) and statistical chart (H). (I, J) Analysis of subcellular distribution of p62 and mitochondria (translocase of outer mitochondrial membrane 20 (Tomm20)) in BV2 cells by confocal microscopy (I) and statistical chart of positive cells (J). All cell experiments were repeated three times, and the values are presented as mean ± standard deviation (SD). ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001. PBS: phosphate-buffered saline; DCA: dichloroacetate; Hpgd: hydroxyprostaglandin dehydrogenase.

Article Snippet: Subsequently, the cells or tissues were treated with the translocase of outer mitochondrial membrane 20 (Tomm20) antibody (1:100; PA5-110506, Thermo Fisher, Waltham, MA, USA) for a second time, followed by an overnight incubation at 4 °C.

Techniques: Activation Assay, Fluorescence, Microscopy, Membrane, Staining, Western Blot, Expressing, Immunofluorescence, Confocal Microscopy, Standard Deviation, Saline